Tietz Textbook of Laboratory Medicine, 8th Edition

SECTION I: BASICS OF LABORATORY MEDICINE
1. Laboratory Medicine
2. Statistical Methodologies in Laboratory Medicine: Analytical and Clinical Evaluation of Laboratory Tests
3. Governance, Risk, and Quality Management in the Medical Laboratory
4. Specimen Collection and Processing
5. Preanalytical Variation and Pre-Examination Processes
6. Quality Control of the Analytical Examination Process
7. Standardization and Harmonization of Analytical Examination Results
8. Biological Variation and Analytical Performance Specifications
9. Establishment and Use of Reference Intervals
10. Evidence-Based Laboratory Medicine
11. Biobanking
12. Laboratory Support of Pharmaceutical, In Vitro Diagnostics, and Epidemiologic Studies
13. Machine Learning and Big Data in Laboratory Medicine
14. Laboratory Stewardship and Test Utilization
15. Principles of Basic Techniques and Laboratory Safety

SECTION II: ANALYTICAL TECHNIQUES
16. Optical Techniques
17. Electrochemistry and Chemical Sensors 18. Electrophoresis 19. Chromatography
20. Mass Spectrometry
21. Sample Preparation for Mass Spectrometry
22. MALDI-TOF Mass Spectrometry Applications in Infectious Diseases
23. Development and Validation of Small Molecule Analytes by Liquid Chromatography-Tandem Mass Spectrometry
24. Proteomics
25. Enzyme and Rate Analysis
26. Immunochemical Techniques
27. Microfabrication and Microfluidics and Their Application in Clinical Diagnostics
28. Cytometry
29. Automation in the Clinical Laboratory
30. Point-of-Care Testing

SECTION III: CLINICAL CHEMISTRY-ANALYTES
31. Amino Acids, Peptides, and Proteins
32. Serum Enzymes
33. Tumor Markers
34. Kidney Function Tests
35. Carbohydrates
36. Lipids and Lipoproteins
37. Electrolytes and Blood Gases
38. Hormones
39. Vitamins and Trace Elements
40. Iron Metabolism
41. Porphyrins and the Porphyrias
42. Therapeutic Drugs and Their Management
43. Clinical Toxicology
44. Toxic Elements
45. Body Fluids

SECTION IV: CLINICAL CHEMISTRY-PATHOPHYSIOLOGY
46. Nutrition: Laboratory and Clinical Aspects
47. Diabetes Mellitus
48. Cardiac Function
49. Kidney Disease
50. Disorders of Water, Electrolytes, and Acid-Base Metabolism
51. Liver Disease
52. Gastric, Intestinal, and Pancreatic Function
53. Monoamine-Producing Tumors
54. Bone and Mineral Metabolism
55. Pituitary Function and Pathophysiology
56. Adrenal Cortex
57. Thyroid Disorders
58. Reproductive Endocrinology and Related Disorders
59. Pregnancy and Its Disorders

SECTION V: GENETIC METABOLIC DISORDERS
60. Newborn Screening and Inborn Errors of Metabolism
61. Inborn Errors of Metabolism: Disorders of Complex Molecules

SECTION VI: MOLECULAR DIAGNOSTICS
62. Principles of Molecular Biology
63. Nucleic Acid Isolation
64. Nucleic Acid Techniques
65. Genomes, Variants, and Massively Parallel Methods
66. Clinical Genome Sequencing
67. Molecular Microbiology
68. Genetics
69. Solid Tumors
70. Hematopathology
71. Circulating Tumor Cells and Circulating Nucleic Acids in Oncology
72. Circulating Nucleic Acids for Prenatal Diagnostics
73. Pharmacogenetics

SECTION VII: HEMATOLOGY AND COAGULATION
74. Automated Hematology
75. Leukocyte Morphology in Blood and Bone Marrow
76. Red Blood Cell Morphology and Indices: The Clinical Chemistry Interface
77. Hemoglobin and Hemoglobinopathies
78. Enzymes of the Red Blood Cell
79. Physiology of Hemostasis
80. Platelets and von Willebrand Factor
81. Coagulation, Anticoagulation, and Fibrinolysis

SECTION VIII: MICROBIOLOGY
82. Introduction to Infectious Diseases
83. The Role of the Clinical Laboratory in Infection Prevention and Antimicrobial Stewardship
84. Bacteriology
85. Antimicrobial Susceptibility Testing
86. Mycobacteriology
87. Mycology
88. Parasitology
89. Virology

SECTION IX: TRANSFUSION MEDICINE
90. Blood Group Systems and Pretransfusion Testing
91. Blood Components, Product Modifications, and Blood Donor Screening
92. Indications for Transfusion: RBCs, Platelets, Plasma, and Cryoprecipitate
93. Transfusion Reactions and Adverse Events Associated With Transfusion

SECTION X: CLINICAL IMMUNOLOGY
94. Systemic Autoimmune Disease
95. Transplant, Solid Organ
96. Hematopoietic Cell Transplantation
97. Transplant Compatibility Testing
98. Monoclonal Antibody Therapeutics and Immunogenicity
99. Allergy Testing
100. Primary Immunodeficiencies and Secondary Immunodeficiencies

APPENDICES
A: Reference Information for the Clinical Laboratory
B: Answers to Multiple Choice Questions

Nader Rifai, PhD, Professor, Department of Pathology Harvard Medical School; The Louis Joseph Gay-Lussac Chair in Laboratory Medicine Boston Children's Hospital; Director of Clinical Chemistry Laboratory Medicine Boston Children's Hospital Boston, Massachusetts, USA